Review



p414 gal1  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    ATCC p414 gal1
    P414 Gal1, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p414+gal1/p414+GAL1/pmc07531523-231-11-12
    Average 90 stars, based on 3 article reviews
    p414 gal1 - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    Plasmid Preparation:

    Article Title: Affinity maturation of antibodies by combinatorial codon mutagenesis versus error-prone PCR
    Article Snippet: PCRs were performed using Q5 High Fidelity DNA polymerase (New England Biolabs, Ipswich, MA) and primers were custom synthesized by Integrated DNA Technologies (Coralville, IA). .. The scFv amplicons were inserted into a linearized plasmid derived from p414-GAL1 (American Type Culture Collection, Manassas, VA) through homologous recombination in S. cerevisiae strain EBY100 (( MATa URA3-52 trp1 leu2Δ1 his3Δ200 pep4::HIS3 prb1Δ1.6 R can1 GAL (pIU211:URA3 ) (American Type Culture Collection, Manassas, VA). .. Yeast cells were conditioned for transformation by 15 min incubation in 2.5 M DTT and transformed by electroporation in 2 mm gap electroporation cuvettes (89047–208, VWR Scientific, Radnor, PA) on a Bio-Rad Gene Pulser II (0.54 kV, 25 uF, resistance set to infinity) in a sucrose containing buffer (10 mM Tris, pH 7.5, 270 mM sucrose, 1 mM MgCl 2 ).

    Derivative Assay:

    Article Title: Affinity maturation of antibodies by combinatorial codon mutagenesis versus error-prone PCR
    Article Snippet: PCRs were performed using Q5 High Fidelity DNA polymerase (New England Biolabs, Ipswich, MA) and primers were custom synthesized by Integrated DNA Technologies (Coralville, IA). .. The scFv amplicons were inserted into a linearized plasmid derived from p414-GAL1 (American Type Culture Collection, Manassas, VA) through homologous recombination in S. cerevisiae strain EBY100 (( MATa URA3-52 trp1 leu2Δ1 his3Δ200 pep4::HIS3 prb1Δ1.6 R can1 GAL (pIU211:URA3 ) (American Type Culture Collection, Manassas, VA). .. Yeast cells were conditioned for transformation by 15 min incubation in 2.5 M DTT and transformed by electroporation in 2 mm gap electroporation cuvettes (89047–208, VWR Scientific, Radnor, PA) on a Bio-Rad Gene Pulser II (0.54 kV, 25 uF, resistance set to infinity) in a sucrose containing buffer (10 mM Tris, pH 7.5, 270 mM sucrose, 1 mM MgCl 2 ).

    Homologous Recombination:

    Article Title: Affinity maturation of antibodies by combinatorial codon mutagenesis versus error-prone PCR
    Article Snippet: PCRs were performed using Q5 High Fidelity DNA polymerase (New England Biolabs, Ipswich, MA) and primers were custom synthesized by Integrated DNA Technologies (Coralville, IA). .. The scFv amplicons were inserted into a linearized plasmid derived from p414-GAL1 (American Type Culture Collection, Manassas, VA) through homologous recombination in S. cerevisiae strain EBY100 (( MATa URA3-52 trp1 leu2Δ1 his3Δ200 pep4::HIS3 prb1Δ1.6 R can1 GAL (pIU211:URA3 ) (American Type Culture Collection, Manassas, VA). .. Yeast cells were conditioned for transformation by 15 min incubation in 2.5 M DTT and transformed by electroporation in 2 mm gap electroporation cuvettes (89047–208, VWR Scientific, Radnor, PA) on a Bio-Rad Gene Pulser II (0.54 kV, 25 uF, resistance set to infinity) in a sucrose containing buffer (10 mM Tris, pH 7.5, 270 mM sucrose, 1 mM MgCl 2 ).



    Similar Products

    p414 gal1  (ATCC)
    90
    ATCC p414 gal1
    P414 Gal1, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p414+gal1/p414+GAL1/pmc07531523-231-11-12
    Average 90 stars, based on 1 article reviews
    p414 gal1 - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    plasmid p414gal1  (ATCC)
    90
    ATCC plasmid p414gal1
    Plasmid P414gal1, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p414+gal1/p414+GAL1/us07651842-273-14-22
    Average 90 stars, based on 1 article reviews
    plasmid p414gal1 - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results